The Dude in The Big Lebowski - I would be shot if I didn't list the memorable performance by Jeff Bridges in the Coen Brothers' comedy classic. John Goodman's Walter Sobchak was pretty good too.
Fred and Barney in The Flintstones - I don't know how those prehistoric chiseled stone bowling balls managed to roll down the alley and knock down those dinosaur pins, but it was always fun to watch the tippy-toe style that the legendary cartoon made famous.
Ralph Kramden in The Honeymooners - How sweet it is! Bowling has never been so hilarious as Ralph pretends to be injured to avoid going to visit his mother-in-law so he can go bowling instead.
Ernie McCraken in Kingpin - Bill Murray is in top form in this often under-appreciated bowling comedy by the Farrelly Brothers that also starred Woody Harrelson and Randy Quaid.
Laverne DeFazio in Laverne and Shirley - Lenny and Squiggy stole the show, but Penny Marshall's classic character was a super bowler.
Al Bundy in Married With Children - Ed O'Neill is the king when it comes to this.
Dan Connor in Roseanne - "The Bowling Episode" is a fan favorite, and John Goodman did a fine job as always.
Homer Simpson in The Simpsons - D'oh!
Clark Kent in Superman III - In an otherwise ho-hum movie sequel, the bowling scenes with Clark and Lana were some of the few highlights.
Are there any favorites of yours that I've left out?